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We leave ours on. However, each computer goes into standby (ie min amount of power) about 2 hours after last mouse/kbd click and REBOOTS at 2am. This clears cache etc and loads certain updates. We don't defrag local disks as our users folders are on the servers. But all the above tips are good - I do them on my home PC's. And you know how bad techies are at looking after their own computers!