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CISCO ME 3400 LAN configuration

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We need a little more info. what is your switch and router configs ?
but until we see more info you may want to see that the switch ports have been assigned the correct VLAN number, and that the PC's firewalls have been configured to respond to pings.

one way to check the firewalls of the PC's is to plug them into an unmanaged switch (no VLANS).
you should be able to ping between the PC's on the same sub-net.