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Performance in network with windows 64 bit server and 32 bit worstations

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I had similar issues in the past on a particular job (after upgrading from Server 2003 32-bit to 2008 64-bit). In the end we found disabling chimney, large send offset and a couple of similar things on the network card did the trick. I can't remember the exact ones as it was too long ago - I think I found the advice in a Microsoft KB article though.