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DataAssist - Data Analysis Software for TaqMan Real-Time PCR Data

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Executive Summary

A data analysis software, DataAssist software, has been developed for quick analysis and interactive visualization of TaqMan real-time PCR data. It uses the comparative CT method to calculate Relative Quantities (RQ) of gene expression for sample comparison. The software uses a refined Grubbs' outlier test to remove outlier among technical replicates, provides a metric to measure control gene stability based on the geNorm algorithm to assist with endogenous control selection, and allows using single or multiple control genes for data normalization.

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