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Microarray data is inherently noisy due to the noise introduced during the preparation of microarray slide. Hence preprocessing of microarray image is an essential to eliminate the background noise in order to enhance the image quality and effective quantification. The objective of this paper is to introduce novel preprocessing techniques such as Optimized Spatial Resolution (OSR) and Spatial Domain Filtering (SDF) for reduction of noise from microarray data and reduction of error during quantification process for estimating the microarray spots accurately to determine expression level of genes.
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