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In this paper, the authors propose a new cross-layer technique that utilizes frequency-domain On-Off Accumulative Transmission (OOAT) in the physical layer to achieve collision-tolerance in the Media Access Control (MAC) layer. The frequency-domain OOAT is developed for wideband systems operating in frequency-selective fading channels. The available spectrum is divided into a large number of orthogonal non-overlapping sub-channels. To achieve collision tolerance, each symbol is transmitted over a set of randomly chosen sub-channels to reduce the probability of collision.