Radio Frequency IDentification technology (RFID) is a non-contact intelligent identification system, its main advantage lies in the wide range of recognition, information capacity, security, excellent resistance to abrasion, reusable. It can take place of the traditional bar code recognition technology, and be applied to the area of modern intelligence logistics management. This paper introduces the working principle of the net three-tier system and RFID technology, and proposes the setting-up of the intelligent logistics system based on three-tier system and RFID technology. It also explains the design and realization of the data access layer, logic layer and presentation layer for providing technical backing to the Logistics management of enterprise monitoring and decision.