Mining Temporal Patterns from iTRAQ Mass Spectrometry(LC-MS/MS) Data

Large-scale proteomic analysis is emerging as a powerful technique in biology and relies heavily on data acquired by state-of-the-art mass spectrometers. As with any other field in Systems Biology, computational tools are required to deal with this ocean of data. iTRAQ (isobaric Tags for Relative and Absolute quantification) is a technique that allows simultaneous quantification of proteins from multiple samples. Although iTRAQ data gives useful insights to the biologist, it is more complex to perform analysis and draw biological conclusions because of its multiplexed design. One such problem is to find proteins that behave in a similar way (i.e. change in abundance) among various time points since the temporal variations in the proteomics data reveal important biological information.

Provided by: National Institutes of Health Topic: Data Management Date Added: Apr 2011 Format: PDF

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