In this paper, efforts have been made to design and simulate the multilayer structured rectangular microstrip antenna for ISM band applications. The shape will provide the bandwidth which is required in various wireless applications like Bluetooth (2.4 GHz-2.484GHz), RFID (2.4GHz-2.5GHz) and WLAN (3.6GHz), etc. Coaxial feed technique is used for its simplicity. The performance of the designed antenna is analyzed in terms of bandwidth, return loss, gain, VSWR, directivity and radiation pattern. FR-4 epoxy substrate has been used, which has dielectric constant of 4.4.