Meander line antenna is small size, low cost, light weight and higher efficiency antenna. Ant Colony Optimization (ACO) is one of the most recent techniques for approximate optimization. The inspiring source of the ACO algorithms is real ant colonies. ACO is inspired by ants' foraging behavior. In this paper, ant colony system algorithm is used to optimize the antenna. Meander line antenna is designed using HFSS tool and Matlab is used to implement ACO algorithm. The structure of antenna is optimized. ACO is best adaptable technique for optimization of meander line antenna.