In present paper, secure online access to enterprises resources is very important for any organization. To protect valuable data become one of the big challenge for today's business as enterprise customers or clients involved in Business-to-Customer (B2C) and Business-to-Business (B2B) e-commerce need to feel that their transactions are secured from system hackers. Biometric technology provides a solution to this problem in enterprise network security. In this paper, the authors enhance the security of online transaction using secure and unique biometric template. The uniqueness of their process is that they store biometric template and password together in encrypted form both without the fusion of score level and decision level, which leads a successful way to combine multiple technologies, different from earlier methods.