Enterprise-level Java applications are ideal candidates for deployment on a virtualized infrastructure using VMware vSphere 4.1. With VMware vSphere 4.1, Java applications can take advantage of the configuration flexibility and consolidation benefits of virtualization while still achieving high levels of performance. This applies to JEE applications deployed in full application-servers, as well as applications deployed on lightweight Web containers such as Tomcat and SpringSource tc Server. In this paper the authors present the results of a performance investigation using a representative enterprise-level Java application on VMware vSphere 4.1. In keeping with recent trends in Java development, they use a Java application which is deployed as a Web application on SpringSource tc Server and which integrates enterprise-level functionality using the Spring framework.