Backing up across a network

Hi,
I recently installed a hard drive on a computer as a fat32.I partioned it so 1 partion is 5 gig and the other is 32 gig.Is there any reason that I would not be able to backup across the network a file from a ntfs partion to the fat32 partioned with 33gig. The file I’m backup is a 17 gig file not compressed.I could backup other files that are approx. 8 gig each with out a problem and there is plenty of room on the 33 gig to back it up on… any help would be appreciated…