on the network, once again with wireshark. A T1 line has more upload bandwidth, but less download bandwidth then DSL (usually), so and trffic thats only download heavy will be slower.
Are you mail and file servers located on the LAN? And are you using samba for file services? Samba does not always play nice with M$ depending on versions.
Also, I would check disk usage and queu on servers to see if its the network or servers.
With out any other info, I would first check email server and file server performance, then check network performance between hosts and servers (ping, trace route), sniff some traffic on the wires in various places, check cables, and then look at WAN, ISP issues. The ISP will always say its your fault right off the bat, so you need to have eliminated any issue at your end first.
[edit should be reply to OP not robo_dev]