Before performing a fdisk/mbr, Check your BIOS boot sequence settings to make sure that the boot drive is listed in the boot sequence. For whatever reason, that may have changed. Check your physical connections e.g. data cabling, power cables and so on. Try the drive on another known working computer. Does the problem follow the drive?
To replace a Master Boot Record on a FAT FAT32 formated primary DOS partition, from a W9X boot disk at the A: prompt type chdir c:\ at the C: prompt type fdisk/mbr this replaces the current master boot record with the W9X default master boot record.