How to make the System 32 folder .

There are some utilities out there to block access to folders.
One is folder guard.

You can hide most of the content of the system32 (and other) windows folders by setting file view options to hide all system and hidden files.

Then, you may want to set a policy to prevent users from changing the settings.

Chas

support.microsoft.com/default.aspx?scid=kb;EN-US;q170086

Basically, this behavior also occurs if there is a String value that contains only a single quote character (") or other invalid dasta as the Value data in the following Registry key:

HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\Windows\CurrentVersion\Run