We could use a little more info on this, but here are a couple suggestions;
Run SYSEDIT and check your CONFIG.SYS & AUTOEXEC.BAT files for references to the drive. If you find any, add REM (and a space) to the front of the line, save the changes and reboot.
In Device Mgr., do you have exclamation marks on anything in Hard Disk Controllers? If so, go to a DOS prompt and type CHKDSK. One of the last lines will be TOTAL BYTES MEMORY. This should be 655,360, except in rare instances. If the number is smaller than this, I would start looking for a virus. Check the floppy used to start the install. If you are using a pirated copy of Windows, that could be infected too.
Does the drive work in DOS?
Do you get errors when trying to use it, either in DOS or Windows? What are they?