Can’t send data across a subnet

I have an large subnet of 10.89.160/21
Allowing ip range of 10.89.160.1 – 10.89.167.254
Gateway of 10.89.160.1
I Can ping from e.g 10.89.162.34 to 10.89.162.60
But can’t ping from 10.89.165.30 to 10.89.163.5 again gateway of 10.89.160.1

Any ideas

Shaggy1405