Internal machine broadcast to the web

i have an ISA server as my proxy, and connects wkstations using firwall client, recently i got a message from our isp that a machine is constantly uploading to the web and which is affecting our bandwidth. i was actually shown the statistic which was captured the cisco router. this broadcast occurs during the work hours. the isa report however did not show any machine as sending broadcast. can this be a virus, and if not, Is there any utility to know which machine is sending this broadcast?