First off....you mention ISA firewall...are you using Microsoft Internet Security and Acceleration server? if so, then you must be running W2K server or W2K Advanced Server with RRAS. Look at Microsoft Tech article 308208. How to Install and Configure a VPN server in Windows 2000.
There are a # of configurations that are required to allow VPN connections depending upon the type of VPN re client to VPN or Router to Router VPN.
Also if your not getting a connection when you use the VPN connection you created via the Network and Dialup connection Wizard, you should receive some type of error such as 673 no answer. This could be a DNS issue.
As I mentioned, there are a # of configuration issues which can cause no connection, so there isn't one simple answer. Please provide more details.
Here are a few other MS tech articles on VPN
320697 How to turn on VPN in SBS server
314067 how to configure a VPN connection in Windows XP
[note: try enabling in and outbound traffic on TCP port 1723]