In this paper, the authors introduce the Bit Error Rate (BER) simulation using 16 QAM (Quadrature Amplitude Modulation) in Matlab. Bit error rate is used to assess systems which can transmit digital data from one address to other address. BER is a unit-less performance measure, often expressed as a percentage. There are few systems where bit error rate applications are useful like fiber optic data systems, radio data link systems, Ethernet, or the systems which transmit data in a network where interference, noise may lower the performance of the digital signal.