Although the web-based bioinformatics is very popular after dozens of years’ growth, biologists found out that it is inconvenient because they need to access many web sites manually in order to perform a single task. Web-service based bioinformatics was proposed to provide well-defined interfaces accessible to programs. However, security for web services is a very important issue that was not addressed in most web-service based bioinformatics systems. The authors developed a Grid-based Secure Web Service Framework for bioinformatics (GSWSF).