Recent development of mobile ad hoc networks, ensembles the nano molecular communication components in order to improve network performance by using Transmitter Nano machine (TN) and Receiver Nano machine (RN). The previous model Nano Ad hoc On-demand Distance Vector (NAODV) routing has the problem higher latency ratio, which claims the lifetime of the network. The authors propose a new Optimized Nano Ad hoc On-demand Distance Vector routing protocol (O-NAODV), which uses the residual energy of mobile nodes while selecting a route. In conventional AODV routing protocol, source node forwards RREQ (Route REQuest) packet to find out path to the destination node.