The first is what is currently in use and what type of Network it is.
The second is what type of Vista you end up with. If it isn't Business then your answer is most likely Yes it will not work at all.
Personally if the entire LAN is running Win 2000 there is absolutely no reason to move to a Business Class System with Vista and lots of reasons not to. If there are buying Business Class Systems XP would be the Perfect fit not offer any problems and work without destroying productivity.
Of course if they are only looking at off The Shelf Domestic Class Systems forget it it will not work as required in the ideal situation and will not work at all if there is a Server with Win 2000 in place as Vista Home Basic Can Not be joined to a Domain.
Col